Scattering and absorption imaging of a highly fractured fluid-filled seismogenetic volume in a region of slow deformation

Regions of slow strain often produce swarm-like sequences, characterized by the lack of a clear mainshock-aftershock pattern. The comprehension of their underlying physical mechanisms is challenging and still debated. We used seismic recordings from the last Pollino swarm (2010–2014) and nearby to separate and map seismic scattering (from P peak-delays) and absorption (from late-time coda-wave attenuation) at different frequencies in the Pollino range and surroundings. High-scattering and high-absorption anomalies are markers of a fluid-filled fracture volume extending from SE to NW (1.5–6 Hz) across the range. With increasing frequency, these anomalies approximately cover the area where the strongest earthquakes occurred from the sixteenth century until 1998. In our interpretation, the NW fracture propagation ends where carbonates of the Lucanian Apennines begin, as marked by a high-scattering and low-absorption area. At the highest frequency (12 Hz) the anomalies widen southward in the middle of the range, consistently marking the faults active during the recent Pollino swarm. Our results suggest that fracture healing has closed small-scale fractures across the SE faults that were active in the past centuries, and that the propagation of fluids may have played a crucial role in triggering the 2010–2014 Pollino swarm. Assuming that the fluid propagation ended at the carbonates barrier in the NW direction, fractures opened new paths to the South, favoring the nucleation of the last Pollino swarm. Indeed, the recently active faults in the middle of the seismogenic volume are marked by a high-scattering and high-absorption footprints. Our work provides evidence that attenuation parameters may track shape and dynamics of fluid-filled fracture networks in fault areas. Keywords: Pollino, Seismic attenuation, Scattering, Fluids, Fractures, Healin

The 1998-1999 Pollino (Southern Apennines, Italy) seismic crisis: tomography of a sequence

In 1998-1999 a seismic sequence occurred in the Southern Apennines, after the moderate size (mb=5.0) 9th September 1998 Pollino earthquake. It lasted about 14 months and was clearly localized to the sole north-west area of the main shock epicenter. Its peculiarity consisted in sudden changes of activity from a series of normal faults with Apenninic (NW-SE) trend and transfer, presumably strike slip, faults with Antiapenninic (NE-SW) and E-W trend. The complexity of the behavior and the different orientations of the activated systems suggest that the area acts as a hinge between the NW-SE trending Southern Apennines and the locally N-S trending Calabrian Arc

Chemical Compositions and Antioxidant Activities of Essential Oils, and Their Combinations, Obtained from Flavedo By-Product of Seven Cultivars of Sicilian Citrus aurantium L.

In this work, seven Citrus aurantium essential oils (EOs) derived from flavedo of cultivars ‘Canaliculata’, ‘Consolei’, ‘Crispifolia’, ‘Fasciata’, ‘Foetifera’, ‘Listata’, and ‘Bizzaria’ were investigated. EOs were also combined in 1:1 (v/v) ratio to identify possible synergism or antagonism of actions. GC-MS analysis was done to investigate Eos’ phytochemical profiles. The antioxidant activity was studied by using a multi-target approach based on FRAP, DPPH, ABTS, and β-carotene bleaching tests. A great difference was observed in EOs’ phytochemical profiles. d-limonene (33.35–89.17%) was the main monoterpene hydrocarbon, and α-Pinene, β-myrcene, and β-linalool were identified in almost all samples. Among EOs, only C3 showed high quantitative and qualitative variability in its chemical composition. The chemical diversity of EOs was also demonstrated by PCA and HCA statistical analysis. Samples C2, C4, C5, C6, and C7 were statistically similar to each other, while C1 and C3 were characterized as having a different amount of other compounds and oxygenated monoterpenes, respectively, with respect to the other EOs mentioned. The global antioxidant score (GAS) revealed that among the tested EOs, C. aurantium ‘Fasciata’ EO had the highest antioxidant potential, with a GAS value of −0.47, whereas among combinations, the EO obtained by mixing ‘Canaliculata’ + ‘Bizzaria’ was the most active. Comparison by theoretical and real data on inhibitory concentration (IC50) and FRAP values did not reveal any significant effect of synergism or antagonism of actions to be valid in all biological applied tests. These findings, considered together, represent an important starting point to understand which compounds are responsible for the activities and their future possible industrial application

Expression and characterization of two new alkane-inducible cytochrome P450s from Trichoderma harzianum

Abstract The inducibility CYPs by various carbon sources, including some n-alkanes and fatty acids, has been studied in Trichoderma harzianum. It was observed that n-dodecane and a mixture of fatty acids were good inducers of total CYP content and ω-hydroxylase of lauric acid, a marker for ω-hydroxylation of n-alkanes. By RACE it was isolated a cDNA containing an open reading frame of 1520 bp which encoded a CYP52 protein of 520 amino acids. Further, another n-alkane inducible CYP was identified in a library of T. harzianum by LC-MS/Ms analysis of a microsomal protein band induced by n-dodecane exposure. Thus, the filamentous fungus T. harzianum is expected to have a CYP dependent conversion of alkanes to fatty acids and their incorporation into cellular lipids

The MASSIMO system for the safeguarding of historic buildings in a seismic area: operationally-oriented platforms

In this paper, the non-invasive system MASSIMO is presented for the monitoring and the seismic vulnerability mitigation of the cultural heritage. It integrates ground-based, airborne and space-borne remote sensing tools with geophysical and in situ surveys to provide the multi-spatial (regional, urban and building scales) and multi-temporal (long-term, short-term, near-real-time and real-time scales) monitoring of test areas and buildings. The measurements are integrated through web-based GIS and 3D visual platforms to support decision-making stakeholders involved in urban planning and structural requalification. An application of this system is presented over the Calabria region for the town of Cosenza and a test historical complex

Analysis of T and NK cell subsets in Sicilian population from young to supercentenarian: the role of age and gender

Ageing dramatically affects number and function of both innate and adaptive arms of immune system, particularly T cell subsets, contributing to reduced vaccination efficacy, decreased resistance to infections and increased prevalence of cancer in the older people. In the present paper, we analysed the age-related changes in the absolute number of lymphocytes in 214 Sicilian subjects, and in the percentages of T and NK cells in a sub-cohort of donors. We compared these results with the immunophenotype of the oldest living Italian supercentenarian (111 years old). The results were also sorted by gender. The correlation between number/percentage of cells and age in all individuals and, separately, in males and females, was examined using a simple linear regression analysis. We did not record the increase in the rate of inversion of the CD4/CD8 ratio frequently reported as associated with ageing in literature. Our observation was the direct consequence of a flat average trend of CD4+ and CD8+ T cell percentages in ageing donors, even when gender differences were included. Our results also suggest that CD4+ and CD8+ subsets are not affected equally by age comparing females with males, and we speculated that gender may affect the response to CMV infection. The supercentenarian showed a unique immunophenotypic signature as regards the relative percentages of her T cell subsets, with CD4+ and CD8+ T cell percentages and CD4+ na\uefve T cell values in line with those recorded for the octogenarian subjects. This suggests that the supercentenarian has a na\uefve "younger" T cell profile comparable to that of a >80 year old female

Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis

: Immunosenescence and inflammaging facilitate the insurgence of chronic diseases. The Mediterranean diet is a non-invasive intervention to improve the chronic low-grade inflammatory status associated with aging. Olive oil oleuropein (OLE) and hydroxytyrosol (HT) demonstrated a controversial modulatory action on inflammation in vitro when tested at concentrations exceeding those detectable in human plasma. We studied the potential anti-inflammatory effects of OLE and HT at nutritionally relevant concentrations on peripheral blood mononuclear cells (PBMCs) as regards cell viability, frequency of leukocyte subsets, and cytokine release, performing an age-focused analysis on two groups of subjects: Adult (age 18–64 years) and Senior (age ≥ 65 years). OLE and HT were used alone or as a pre-treatment before challenging PBMCs with lipopolysaccharide (LPS). Both polyphenols had no effect on cell viability irrespective of LPS, but 5 µM HT had an LPS-like effect on monocytes, reducing the intermediate subset in Adult subjects. OLE and HT had no effect on LPS-triggered release of TNF-α, IL-6 and IL-8, but 5 µM HT reduced IL-10 secretion by PBMCs from Adult vs. Senior group. In summary, nutritionally relevant concentrations of OLE and HT elicit no anti-inflammatory effect and influence the frequency of immune cell subsets with age-related different outcomes

Effects of a commercially available branched-chain amino acid-alanine-carbohydrate-based sports supplement on perceived exertion and performance in high intensity endurance cycling tests

Background:Sports nutritional supplements containing branched-chain amino acids (BCAA) have been widelyreported to improve psychological and biological aspects connected to central fatigue and performance inendurance exercise, although the topic is still open to debate. The aim of the present study was to determinewhether the intake of a commercially available BCAA-based supplement, taken according to the manufacturer’srecommendations, could affect the rating of perceived exertion (RPE) and performance indexes at the beginning(1d) and end of a 9-week (9w) scheduled high intensity interval training program, with an experimental approachintegrating the determination of psychometric, performance, metabolic and blood biochemical parameters.Methods:This was a randomized double-blind placebo-controlled study. Thirty-two untrained, healthy young adults(20 males and 12 female) were enrolled. A high-intensity endurance cycling (HIEC) test was used to induce fatigue inthe participants: HIEC consisted in ten 90 s sprints interspersed by ten 3 min recovery phases and followed by a finalstep time to exhaustion was used. In parallel with RPE, haematological values (creatine kinase, alanine, BCAA,tryptophan, ammonia and glucose levels), and performance indexes (maximal oxygen consumption - VO2max,powerassociated with lactate thresholds - WLT1,WLT2and time to exhaustion - TTE) were assessed. All subject took thesupplement (13.2 g of carbohydrates; 3.2 g of BCAA and 1.6 g of L-alanine per dose) or placebo before each test andtraining session. Dietary habits and training load were monitored during the entire training period.Results:The administration of the supplement (SU) at 1d reduced RPE by 9% during the recovery phase, as comparedto the placebo (PL); at 9w the RPE scores were reduced by 13 and 21% during the sprint and recovery phase,respectively; at 9w, prolonged supplement intake also improved TTE and TRIMP. SU intake invariably promoted a rapidincrease (within 1 h) of BCAA serum blood levels and prevented the post-HIEC tryptophan: BCAA ratio increase foundin the PL group, at both 1d and 9w. There was no difference in dietary habits between groups and those habits didnot change over time; no difference in glycemia was found between SU and PL. VO2max,WLT1and WLT2valuesimproved over time, but were unaffected by supplement intake. Conclusions:On the whole, these results suggest that i) the intake of the BCAA-based commercially availablesupplement used in this study reduces RPE as a likely consequence of an improvement in the serum tryptophan: BCAAratio; ii) over time, reduced RPE allows subjects to sustain higher workloads, leading to increased TRIMP and TTE

A model for the cosmological evolution of low frequency radio sources

We present a new evolutionary model that describes the population properties of radio sources at frequencies <5 GHz, thus complementing the De Zotti et al. (2005) model, holding at higher frequencies. We find that simple analytic luminosity evolution is still sufficient to fit the wealth of available data on local luminosity functions, multi-frequency source counts, and redshift distributions. However, the fit requires a luminosity-dependent decline of source luminosities at high redshifts, at least for steep-spectrum sources, thus confirming earlier indications of a "downsizing" also for radio sources. The upturn of source counts at sub-mJy levels is accounted for by a straightforward extrapolation, using the empirical far-IR/radio correlation, of evolutionary models matching the far-IR counts and redshift distributions of star-forming galaxies. We also discuss the implications of the new model for the interpretation of data on large-scale clustering of radio sources and on the Integrated Sachs-Wolfe (ISW) effect, and for the investigation of the contribution of discrete sources to the extragalactic background. As for the ISW effect, a new analysis exploiting a very clean CMB map, yields at a substantially higher significance than reported before.Comment: 14 pages, 11 figures, accepted for publication on MNRA

The Monitoring of Urban Environments and Built-Up Structures in a Seismic Area: Web-Based GIS Mapping and 3D Visualization Tools for the Assessment of the Urban Resources

In this paper, a non-invasive infrastructural system called MASSIMO is presented for the monitoring and the seismic vulnerability mitigation of cultural heritages. It integrates ground-based, airborne and space-borne remote sensing tools with geophysical and in situ surveys to provide a multi-spatial (regional, urban and building scales) and multi-temporal (longterm, short-term and near-real-time scales) monitoring of test areas and buildings. The measurements are integrated through web-based Geographic Information System (GIS) and 3-dimensional visual platforms to support decision-making stakeholders involved in urban and structural requalification planning. An application of this system is presented over the Calabria region for the town of Cosenza and a test historical complex.The present work is supported and funded by the Italian Ministry of Education, University and Research (MIUR) under the research project PON01-02710 "MASSIMO" - "Monitoraggio in Area Sismica di SIstemi MOnumentali".Published9-134T. Sismologia, geofisica e geologia per l'ingegneria sismicaN/A or not JC